Description
Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TIMP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-3 can be calculated.
Background: Metalloproteinase inhibitor 3 is an protein that in humans is encoded by the TIMP3 gene, which maps to to chromosome 22. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. The balance of MMP and TIMP determines the net migratory capacity of DCs. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy